Development of a cloud point extraction and spectrophotometry-based microplate method for the determination of nitrite in human urine and blood.
Identifieur interne : 000552 ( Main/Exploration ); précédent : 000551; suivant : 000553Development of a cloud point extraction and spectrophotometry-based microplate method for the determination of nitrite in human urine and blood.
Auteurs : Jiao Zhao [République populaire de Chine] ; Yunhui Lu [République populaire de Chine] ; Chongyang Fan [République populaire de Chine] ; Jun Wang [États-Unis] ; Yaling Yang [République populaire de Chine]Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [ 1873-3557 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Nitrites.
- chemical , chemistry : Polyethylene Glycols, Surface-Active Agents.
- instrumentation : Chemical Fractionation.
- methods : Chemical Fractionation, Spectrophotometry.
- chemical , urine : Nitrites.
- Equipment Design, Humans, Limit of Detection.
Abstract
A novel and simple method for the sensitive determination of trace amounts of nitrite in human urine and blood has been developed by combination of cloud point extraction (CPE) and microplate assay. The method is based on the Griess reaction and the reaction product is extracted into nonionic surfactant Triton-X114 using CPE technique. In this study, decolorization treatment of urine and blood was applied to overcome the interference of matrix and enhance the sensitivity of nitrite detection. Multi-sample can be simultaneously detected thanks to a 96-well microplate technique. The effects of different operating parameters such as type of decolorizing agent, concentration of surfactant (Triton X-114), addition of (NH4)2SO4, extraction temperature and time, interfering elements were studied and optimum conditions were obtained. Under the optimum conditions, a linear calibration graph was obtained in the range of 10-400 ng mL(-1) of nitrite with limit of detection (LOD) of 2.5 ng mL(-1). The relative standard deviation (RSD) for determination of 100 ng mL(-1) of nitrite was 2.80%. The proposed method was successfully applied for the determination of nitrite in the urine and blood samples with recoveries of 92.6-101.2%.
DOI: 10.1016/j.saa.2014.09.097
PubMed: 25448978
Affiliations:
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The method is based on the Griess reaction and the reaction product is extracted into nonionic surfactant Triton-X114 using CPE technique. In this study, decolorization treatment of urine and blood was applied to overcome the interference of matrix and enhance the sensitivity of nitrite detection. Multi-sample can be simultaneously detected thanks to a 96-well microplate technique. The effects of different operating parameters such as type of decolorizing agent, concentration of surfactant (Triton X-114), addition of (NH4)2SO4, extraction temperature and time, interfering elements were studied and optimum conditions were obtained. Under the optimum conditions, a linear calibration graph was obtained in the range of 10-400 ng mL(-1) of nitrite with limit of detection (LOD) of 2.5 ng mL(-1). The relative standard deviation (RSD) for determination of 100 ng mL(-1) of nitrite was 2.80%. The proposed method was successfully applied for the determination of nitrite in the urine and blood samples with recoveries of 92.6-101.2%.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Jiao" sort="Zhao, Jiao" uniqKey="Zhao J" first="Jiao" last="Zhao">Jiao Zhao</name></noRegion><name sortKey="Fan, Chongyang" sort="Fan, Chongyang" uniqKey="Fan C" first="Chongyang" last="Fan">Chongyang Fan</name><name sortKey="Lu, Yunhui" sort="Lu, Yunhui" uniqKey="Lu Y" first="Yunhui" last="Lu">Yunhui Lu</name><name sortKey="Yang, Yaling" sort="Yang, Yaling" uniqKey="Yang Y" first="Yaling" last="Yang">Yaling Yang</name></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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